Journal: AJR. American journal of roentgenology

Article Title: Prognostic Value of Pretreatment MRI in Patients With Prostate Cancer Treated With Radiation Therapy: A Systematic Review and Meta-Analysis

doi: 10.2214/AJR.19.21836

Figure Lengend Snippet: Radiation Therapy (RT) and MRI Characteristics

Article Snippet: Jackson [ 30 ] , E , 60–74 , All , Siemens , GBS1, Vision , 1.5 , No , T2-weighted imaging , 1 , NA , Consultant.

Techniques: Imaging

Journal: Science Advances

Article Title: Oral polyphenol-armored nanomedicine for targeted modulation of gut microbiota–brain interactions in colitis

doi: 10.1126/sciadv.adf3887

Figure Lengend Snippet: ( A ) Synthetic scheme of siRNA-GBSA(CHI/TA) n NPs. ( B ) siRNA-GBSA(CHI/TA) 5 NPs were orally administered by gavage and passed smoothly through the extreme stomach environment under the protection of (CHI/TA) 5 armor. The armor slowly degraded at the intestinal site, enriching the inflamed colon through electrostatic adsorption and polyphenol adhesion. ( C ) The microenvironment of the inflamed colon site was regulated by relieving the inflammatory responses and alleviating the imbalance of intestinal flora by siRNA-GBSA(CHI/TA) 5 NPs. Moreover, inflammation in the brains of IBD mice was also alleviated via the gut-brain axis and accompanied by decreased activation of astrocytes, microglia, and neuroinflammatory cells in the brain. Consequently, the relief of brain inflammation improved cognitive performance.

Article Snippet: GQD, GAGQD, GBSA NPs, GBSA(CHI/TA) 5 NPs, and the reagents provided in the kit were added to the 96-well plate according to the manufacturer’s instructions, with a total volume of 200 μl per well.

Techniques: Adsorption, Activation Assay

Journal: Science Advances

Article Title: Oral polyphenol-armored nanomedicine for targeted modulation of gut microbiota–brain interactions in colitis

doi: 10.1126/sciadv.adf3887

Figure Lengend Snippet: ( A ) TEM images and particle size distribution maps of GAGQD. ( B ) HRTEM of GAGQD. ( C ) High-resolution x-ray photoelectron spectroscopy data of C1s for GAGQD. ( D ) UV-vis absorption spectra of GQD and GAGQD. a.u., arbitrary units. ( E ) Scanning electron microscopy (SEM) images of siRNA-GBSA(CHI/TA) 5 NPs. ( F and G ) Encapsulation efficiency of siRNA or GAGQD in siRNA-GBSA NPs. ( H and I ) •O 2 − elimination activity and •OH elimination activity of GBSA NPs and GBSA(CHI/TA) 5 NPs. ( J and K ) •O 2 − elimination activity and •OH elimination activity of GQD and GAGQD. (Data are presented as the mean ± SD; * P < 0.05, ** P < 0.01, and **** P < 0.0001).

Article Snippet: GQD, GAGQD, GBSA NPs, GBSA(CHI/TA) 5 NPs, and the reagents provided in the kit were added to the 96-well plate according to the manufacturer’s instructions, with a total volume of 200 μl per well.

Techniques: Spectroscopy, Electron Microscopy, Encapsulation, Activity Assay

Journal: Science Advances

Article Title: Oral polyphenol-armored nanomedicine for targeted modulation of gut microbiota–brain interactions in colitis

doi: 10.1126/sciadv.adf3887

Figure Lengend Snippet: ( A ) Diagram showing how siRNA-GBSA(CHI/TA) 5 NPs resist degradation in the gastrointestinal tract and eventually accumulate in the colon. ( B ) SEM images of siRNA-GBSA NPs, siRNA-GBSA(CHI/TA) 2 NPs, and siRNA-GBSA(CHI/TA) 5 NPs after incubation in simulated gastric fluid (SGF) for 2 hours, simulated intestinal fluid (SIF) for 4 hours, and simulated colonic fluid (SCF) for 4 and 12 hours. Scale bars, 1 μm. ( C ) In vivo small animal fluorescence imaging of normal and IBD mice (drinking 3% DSS sterile water for 3 days) at different time points (3, 6, 12, and 24 hours) after intragastric administration of Cy5.5, Cy5.5-BSA NPs, and Cy5.5-BSA(CHI/TA) 5 NPs. ( D ) In vitro fluorescence images of colons after gavage of Cy5.5, Cy5.5-BSA NPs, or Cy5.5-BSA(CHI/TA) 5 NPs for 24 hours. ( E and F ) Corresponding fluorescence (FL) quantitative statistics of in vivo fluorescence imaging of mice and in vitro fluorescence of colon after 24 hours of intragastric administration. ( G ) In vitro fluorescence images and ( H ) fluorescence quantitative analysis of mouse colons cultured with Cy5.5-BSACHI NPs or Cy5.5-BSA(CHI/TA) 5 NPs for 1 hour, following three washes. (Data are presented as the mean ± SD; * P < 0.05 and ** P < 0.01).

Article Snippet: GQD, GAGQD, GBSA NPs, GBSA(CHI/TA) 5 NPs, and the reagents provided in the kit were added to the 96-well plate according to the manufacturer’s instructions, with a total volume of 200 μl per well.

Techniques: Incubation, In Vivo, Fluorescence, Imaging, Sterility, In Vitro, Cell Culture

Journal: Science Advances

Article Title: Oral polyphenol-armored nanomedicine for targeted modulation of gut microbiota–brain interactions in colitis

doi: 10.1126/sciadv.adf3887

Figure Lengend Snippet: ( A ) Experimental schedule. siRNA-GBSA(CHI/TA) 5 NPs were administered on days 2 to 7, and all mice underwent behavioral testing on the indicated days. ( B ) Forced swim test. (i) Schematic of forced swim test, (ii) immobility time, and (iii) latency to first immobility in the forced swim test. ( C ) Tail suspension test. (i) Schematic of tail suspension test, (ii) immobility time, and (iii) latency to first immobility of the tail suspension test. ( D ) The beam walk test. (i) Schematic of the beam walk test; (ii) latency to cross the beam. ( E ) The open field test. (i) Typical escape route map of each group in the open field test; (ii) total distance of each group in the open field test. ( F ) Novel object recognition test. (i) Schematic of novel object recognition test; (ii) exploration time of a new object in the new object recognition test. ( G ) MWM test. (i) Schematic of MWM test, (ii) typical escape route map of each group in MWM test, and (iii) time of entering the T zone for the first time in MWM test. ( n = 10 mice per group; Data are presented as the mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001).

Article Snippet: GQD, GAGQD, GBSA NPs, GBSA(CHI/TA) 5 NPs, and the reagents provided in the kit were added to the 96-well plate according to the manufacturer’s instructions, with a total volume of 200 μl per well.

Techniques: Suspension

Journal: Journal of Biomolecular Structure & Dynamics

Article Title: Natural-like products as potential SARS-CoV-2 M pro inhibitors: in-silico drug discovery

doi: 10.1080/07391102.2020.1790037

Figure Lengend Snippet: Decomposition of MM-GBSA binding energies for four dioxolo-derivatives in complex with SARS-CoV-2 main protease (M pro ) through 50 ns MD simulations.

Article Snippet: Therefore, MM-GBSA binding energy was estimated per-frame for each of MolPort-004-849-765, MolPort-000-708-794, MolPort-002-513-915, and MolPort-000-702-646 with M pro and presented in .

Techniques: Binding Assay